ERC BOOST project is on the cover!

New biomaterials for bone tissue engineering (BTE) applications need to be tested in a reliable model of bone microenvironment, and in vitro tests with bone cells represent worthy systems.

In the paper "Protocol of Co-Culture of Human Osteoblasts and Osteoclasts to Test Biomaterials for Bone Tissue Engineering" of G. Borciani et al. recently published in the journal Methods and Protocols, a clear protocol to set up an indirect co-culture system of human-derived osteoblasts (OBs) and osteoclasts (OCs), reporting cell seeding density, cell:cell ratio, culture medium, and proofs of differentiation, is provided. The material to be tested is easily introduced in the system and the physical separation of OBs and OCs allows distinguishing the effects of the material on the two cell types via direct/indirect interaction between material and cells. The system is an in vitro model of bone coupling, useful in testing innovative materials for bone regeneration, and potentially exploitable in different application fields.

Get a look: https://www.mdpi.com/2409-9279/5/1/8